Anti-Histone H3 (di methyl K4) antibody (ab272142)
Key features and details
- Rabbit polyclonal to Histone H3 (di methyl K4)
- Suitable for: ChIP, ICC/IF, WB, Dot blot
- Reacts with: Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (di methyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (di methyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, ICC/IF, WB, Dot blotmore details -
Species reactivity
Reacts with: Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (di methyl K4).
Database link: Q71DI3 -
Positive control
- ICC: Caenorhabditis elegans embryo. WB: Caenorhabditis elegans embryo lysate, HeLa histone preparation. ChIP: Chromatin from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 antibody (ab272142)
Immunofluorescence analysis of Caenorhabditis elegans embryo stained for Histone H3 (green) using ab272142 at 1:50, followed by a Dylight 488 secondary antibody at 1:10,000. Localization: Histone H3 [Dimethyl Lys4] is nuclear and chromosomal. Counterstaining of nuclei with DAPI (blue). Fixation: 0.5% PFA. Antigen retrieval: Not required.
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ChIP - Anti-Histone H3 antibody (ab272142)Chromatin Immunoprecipitation of ab272142. Chromatin from one million formaldehyde cross-linked HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was used with 2ug of ab272142 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one. -
Western blot - Anti-Histone H3 antibody (ab272142)Anti-Histone H3 (di methyl K4) antibody (ab272142) at 1/500 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone preparation at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO
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Dot Blot - Anti-Histone H3 antibody (ab272142)Dot Blot analysis of ab272142 at 1:1000, followed by Dylight™488 rabbit secondary antibody at 1:10,000. Block: 5% BLOTTO.
Lane 1: K4.
Lane 2: K4 Kme1.
Lane 3: K4 Kme2.
Lane 4: K4 Kme3.
Lane 5: K4 KAc.
Load: 1, 3, 10, and 25 picomoles of peptide.
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Western blot - Anti-Histone H3 antibody (ab272142)Anti-Histone H3 (di methyl K4) antibody (ab272142) at 1/500 dilution + Caenorhabditis elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDaBlock: 5% BLOTTO