Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)
![Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)](https://www.abcam.com/ps/products/271/ab271346/Images/ab271346-384444-rabbit-monoclonal-antibody-rm339-dotblot-crotonylated-histone-h3.png "Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM339] to Histone H3 (crotonyl K9)
- Suitable for: WB, Dot blot
- Reacts with: Recombinant fragment
Overview
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Product name
Anti-Histone H3 (crotonyl K9) antibody [RM339]
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [RM339] to Histone H3 (crotonyl K9) -
Host species
Rabbit -
Specificity
No cross reactivity with other crotonylated or acetylated Lysines in histone H3.
Cross reactivity is seen with Histone H3 butyrylated at Lysine 9.
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Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Histone H3 (crotonyl K9).
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Positive control
- Dot Blot: Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl) peptide. WB: Histone H3 crotonylated at Lysine 9 (H3K9-Crotonyl) peptide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM339 -
Isotype
IgG
Images
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Dot Blot - Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)
Dot Blot - Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)
Shows ab271346 reacts specifically to Histone H3 crotonylated at Lysine 9.
Cross reactivity is seen with Histone H3 butyrylated at Lysine 9.
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![Western blot - Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)](https://www.abcam.com/ps/products/271/ab271346/Images/ab271346-384443-rabbit-monoclonal-antibody-rm339-wb-crotonylated-histone-h3.png)
Western blot - Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)Western blot - Anti-Histone H3 (crotonyl K9) antibody [RM339] ab271346 at 5 µg/ml.
Samples: H3K9cr [Crotonyl-Histone H3 (Lys9)].
Controls: Anti-Histone H3 and anti-G6PDH.
A crotonylation inducing metabolite was used to increase the H3K9cr signal.
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![Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)](https://www.abcam.com/ps/products/271/ab271346/Images/ab271346-3-benefits-of-recombinant-antibodies.png)
Anti-Histone H3 (crotonyl K9) antibody [RM339] (ab271346)