Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab12181)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K9, phospho S10)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (acetyl K9, phospho S10) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K9, phospho S10) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography. The resulting IgG fraction is further purified by absorption on the unmodified histone H3 peptide (human, amino acids 7-20). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab12181)This image was kindly supplied as part of the review submitted by Monika Harrer.
-
Western blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab12181)All lanes : Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab12181)
Lane 1 : Whole cell extract of NIH3T3 cells
Lane 2 : Whole cell extract of NIH3T3 cells with [Ac-Lys9, pSer10] Histone H3 peptide
Lane 3 : Whole cell extract of NIH3T3 cells with [non-Ac, non-phos] Histone H3 peptide
Lane 4 : Whole cell extract of NIH3T3 cells with [Ac-Lys9] Histone H3 peptide
Lane 5 : Whole cell extract of NIH3T3 cells with [pSer10] Histone H3 peptide
Secondary
All lanes : Alkaline phosphatase conjugated Goat anti-Rabbit developed with a colorimetric substrate
Observed band size: 17 kDa why is the actual band size different from the predicted?
Inhibition is shown in Lane 2.
