Anti-HCP1/PCFT antibody (ab25134)
Key features and details
- Rabbit polyclonal to HCP1/PCFT
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-HCP1/PCFT antibody
See all HCP1/PCFT primary antibodies -
Description
Rabbit polyclonal to HCP1/PCFT -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody has 80% homology with the human HCP1 / PCFT protein. Some customers have successfully used ab25134 in human tissue. Please contact Abcam Scientific Support for more information. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide corresponding to Mouse HCP1/PCFT aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab25847) -
General notes
Previously labelled as HCP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). Target
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Function
Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme. -
Tissue specificity
Expressed in kidney, liver, placenta, small intestine, spleen, retina and retinal pigment epithelium. Lower levels found in colon and testis. Very low levels in brain, lung, stomach, heart and muscle. In intestine, expressed in duodenum with lower levels in jejunum, ileum, cecum, rectum and segments of the colon. -
Involvement in disease
Defects in SLC46A1 are the cause of hereditary folate malabsorption (HFM) [MIM:229050]. HFM is a rare autosomal recessive disorder characterized by impaired intestinal folate absorption with folate deficiency resulting in anemia, hypoimmunoglobulinemia with recurrent infections, and recurrent or chronic diarrhea. In many patients, neurological abnormalities such as seizures or mental retardation become apparent during early childhood, attributed to impaired transport of folates into the central nervous system. When diagnosed early, the disorder can be treated by administration of folate. If untreated, it can be fatal and, if treatment is delayed, the neurological defects can become permanent. -
Sequence similarities
Belongs to the major facilitator superfamily. SLC46A family. -
Cellular localization
Apical cell membrane. Cytoplasm. Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in the cytoplasm in iron-replete cells. - Information by UniProt
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Database links
- Entrez Gene: 113235 Human
- Entrez Gene: 52466 Mouse
- Entrez Gene: 303333 Rat
- Omim: 611672 Human
- SwissProt: Q96NT5 Human
- SwissProt: Q6PEM8 Mouse
- SwissProt: Q5EBA8 Rat
- Unigene: 131618 Mouse
see all -
Alternative names
- G21 antibody
- HCP 1 antibody
- HCP1 antibody
see all
Images
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Western blot - Anti-HCP1/PCFT antibody (ab25134)All lanes : Anti-HCP1/PCFT antibody (ab25134) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Spleen (Rat) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
Western blot - Anti-HCP1/PCFT antibody (ab25134)All lanes : Anti-HCP1/PCFT antibody (ab25134) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-HCP1/PCFT antibody (ab25134)ICC/IF image of ab25134 stained NIH-3T3 cells. The cells were 4% paraformladhyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25134, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (10)
ab25134 has been referenced in 10 publications.
- Alam C et al. Upregulation of reduced folate carrier by vitamin D enhances brain folate uptake in mice lacking folate receptor alpha. Proc Natl Acad Sci U S A 116:17531-17540 (2019). PubMed: 31405972
- Sid V et al. High-fat diet consumption reduces hepatic folate transporter expression via nuclear respiratory factor-1. J Mol Med (Berl) 96:1203-1213 (2018). PubMed: 30178194
- Li H et al. Physiological stress-induced corticosterone increases heme uptake via KLF4-HCP1 signaling pathway in hippocampus neurons. Sci Rep 7:5745 (2017). PubMed: 28720846
- Samaniego R et al. Macrophage uptake and accumulation of folates are polarization-dependent in vitro and in vivo and are regulated by activin A. J Leukoc Biol 95:797-808 (2014). PubMed: 24399840
- Takada T et al. Selective accumulation of hematoporphyrin derivative in glioma through proton-coupled folate transporter SLC46A1. J Clin Biochem Nutr 54:26-30 (2014). IHC . PubMed: 24426187
- Cao C et al. Duodenal absorption and tissue utilization of dietary heme and nonheme iron differ in rats. J Nutr 144:1710-7 (2014). PubMed: 25332470
- Panda D et al. RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses. Proc Natl Acad Sci U S A 108:19036-41 (2011). WB ; Human . PubMed: 22065774
- Solanky N et al. Expression of folate transporters in human placenta and implications for homocysteine metabolism. Placenta 31:134-43 (2010). PubMed: 20036773
- Schaer CA et al. Heme carrier protein (HCP-1) spatially interacts with the CD163 hemoglobin uptake pathway and is a target of inflammatory macrophage activation. J Leukoc Biol 83:325-33 (2008). PubMed: 17947394
- Subramanian VS et al. Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine. Am J Physiol Cell Physiol 295:C828-35 (2008). PubMed: 18650265
Images
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Western blot - Anti-HCP1/PCFT antibody (ab25134)All lanes : Anti-HCP1/PCFT antibody (ab25134) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Spleen (Rat) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
-
Western blot - Anti-HCP1/PCFT antibody (ab25134)All lanes : Anti-HCP1/PCFT antibody (ab25134) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-HCP1/PCFT antibody (ab25134)ICC/IF image of ab25134 stained NIH-3T3 cells. The cells were 4% paraformladhyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25134, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
