Anti-Lin28B antibody (ab115698)
Key features and details
- Rabbit polyclonal to Lin28B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lin28B antibody
See all Lin28B primary antibodies -
Description
Rabbit polyclonal to Lin28B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH from a region corresponding to the N-terminal of Human Lin28B.
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Positive control
- Human spleen and testis tissues and HL60 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lin28B antibody (ab115698)Anti-Lin28B antibody (ab115698) at 1/1000 dilution + HL60 cell line lysate at 35 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/5000 dilution
Predicted band size: 27 kDaIncubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (ab115698)Immunohistochemistry Image of Human Spleen (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (ab115698)Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28B antibody (ab115698)Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Flow Cytometry - Anti-Lin28B antibody (ab115698)Overlay histogram showing HL-60 cells stained with ab115698 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab115698, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.
