Anti-Lin28B antibody (ab264333)
Key features and details
- Rabbit polyclonal to Lin28B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lin28B antibody
See all Lin28B primary antibodies -
Description
Rabbit polyclonal to Lin28B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Lin28B aa 1-50. The exact sequence is proprietary. NP_001004317.1
Database link: Q6ZN17 -
Positive control
- WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lin28B antibody (ab264333)All lanes : Anti-Lin28B antibody (ab264333) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen
) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Lin28B antibody (ab264333)Lin28B was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264333 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab264333 at 1 μg/ml.
Lane 1: ab264333 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 3 seconds.
