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Signal Transduction Antibodies ha tag

Anti-HA tag antibody (ab13834)

Price and availability

268 032 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-HA tag antibody (ab13834)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HA tag
  • Suitable for: WB
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-HA tag antibody
    See all HA tag primary antibodies
  • Description

    Rabbit polyclonal to HA tag
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide within HA tag conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab13835)

  • Positive control

    • WB: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • Alternative Versions

    • HRP Anti-HA tag antibody (ab128131)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • HA tag peptide (ab13835)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500. Can be blocked with HA tag peptide (ab13835).
Notes
WB
1/500. Can be blocked with HA tag peptide (ab13835).

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Western blot - Anti-HA tag antibody (ab13834)
    Western blot - Anti-HA tag antibody (ab13834)
    All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

    Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
    Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab13834? Please let us know so that we can cite the reference in this datasheet.

    ab13834 has been referenced in 7 publications.

    • Wang C  et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). PubMed: 31076507
    • Chen X  et al. Deficiency or inhibition of lysophosphatidic acid receptor 1 protects against hyperoxia-induced lung injury in neonatal rats. Acta Physiol (Oxf) 216:358-75 (2016). PubMed: 26495902
    • Myers SA  et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. Elife 5:e10647 (2016). PubMed: 26949256
    • Huang Y  et al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB ; Human . PubMed: 21307340
    • Alhamidi M  et al. Fukutin-Related Protein Resides in the Golgi Cisternae of Skeletal Muscle Fibres and Forms Disulfide-Linked Homodimers via an N-Terminal Interaction. PLoS One 6:e22968 (2011). WB . PubMed: 21886772
    • Reszka N  et al. Simian TRIM5alpha proteins reduce replication of herpes simplex virus. Virology 398:243-50 (2010). PubMed: 20060996
    • Ichimura K  et al. IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas. Neuro Oncol 11:341-7 (2009). WB . PubMed: 19435942

    Images

    • Western blot - Anti-HA tag antibody (ab13834)
      Western blot - Anti-HA tag antibody (ab13834)
      All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

      Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
      Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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