Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-V5 tag antibody [E10] (ab53418)

Key features and details

  • Mouse monoclonal [E10] to V5 tag
  • Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-V5 tag antibody [E10]
    See all V5 tag primary antibodies

  • Description

    Mouse monoclonal [E10] to V5 tag

  • Host species

    Mouse

  • Specificity

    ab53418 detects (GKPIPNPLLGLDST) V5 tag fusion protein.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide:

    GKPIPNPLLGLDST

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • 293 cells untransfected and transfected with lacZ-V5-tagged.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab53418 was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.

  • Clonality

    Monoclonal

  • Clone number

    E10

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (ab53418)
    Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (ab53418) This image is courtesy of an Abreview submitted by Javier Rodriguez

    ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.

    See Abreview

  • Western blot - Anti-V5 tag antibody [E10] (ab53418)
    Western blot - Anti-V5 tag antibody [E10] (ab53418)
    All lanes : Anti-V5 tag antibody [E10] (ab53418) at 1/1000 dilution

    Lane 1 : 293 cells
    untransfected (control)
    Lane 2 : 293 cells
    transfected
    with lacZ-V5-tagged.

    Lysates/proteins at 30 µg per lane.

    Observed band size: 130 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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