Antibodies, Proteins, Kits and Reagents for Life Science

358 492 ₸ Product size

50 µl 358 492 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [HA-7] to HA tag
Suitable for: ELISA, ICC/IF, IP, WB
Reacts with: Species independent
Isotype: IgG1

Product name

Anti-HA tag antibody [HA-7]
See all HA tag primary antibodies
Description

Mouse monoclonal [HA-7] to HA tag
Host species

Mouse
Tested applications

Suitable for: ELISA, ICC/IF, IP, WBmore details
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide:
YPYDVPDYA
conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag
Run BLAST with Run BLAST with
Positive control
- Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
General notes

This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Proprietary Purification
Purification notes

Purified from Tissue culture supernatant”.
Clonality

Monoclonal
Clone number

HA-7
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)

HEK-293 cells overexpressing N-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoprecipitation - Anti-HA tag antibody [HA-7] (ab49969) This image is courtesy of an anonymous Abreview

ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA-7] (ab49969)

HEK-293 cells overexpressing C-terminal HA tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1 μg/mL Anti-HA tag antibody [HA-7] (ab49969). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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