Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18992] to RFP
Suitable for: ICC/IF, WB
Reacts with: Mouse, Human

Product name

Anti-RFP antibody [EPR18992]
See all RFP primary antibodies
Description

Rabbit monoclonal [EPR18992] to RFP
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to RFP aa 1 to the C-terminus. (Entacmaea quadricolor)
Database link: Q8ISF8

Run BLAST with Run BLAST with
Positive control
- WB: RFP transfected HEK-293 whole cell lysate. ICC/IF: RFP transfected HEK-293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18992
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody [EPR18992] (ab185921)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Empty vector transfected or RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RFP with ab185921 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing positive staining after transfection of RFP on HEK-293 cells.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [EPR18992] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab185921 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Western blot - Anti-RFP antibody [EPR18992] (ab185921)

All lanes : Anti-RFP antibody [EPR18992] (ab185921) at 1/10000 dilution

Lane 1 : RFP transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Empty vector (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : Human kidney lysate
Lane 6 : Human liver lysate
Lane 7 : Mouse brain lysate
Lane 8 : Mouse heart lysate
Lane 9 : Rat brain lysate
Lane 10 : Rat heart lysate
Lane 11 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 12 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 14 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-RFP antibody [EPR18992] (ab185921)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com