Anti-RFP antibody [RF5R] (ab125244)
![Anti-RFP antibody [RF5R] (ab125244)](https://www.abcam.com/ps/products/125/ab125244/Images/ab125244-139414-anti-rfp-antibody-rf5r-western-blot.jpg "Anti-RFP antibody [RF5R] (ab125244)")
Key features and details
- Mouse monoclonal [RF5R] to RFP
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-RFP antibody [RF5R]
See all RFP primary antibodies -
Description
Mouse monoclonal [RF5R] to RFP -
Host species
Mouse -
Specificity
Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Transfected HEK293 cell lysate.
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General notes
Store at 4°C (add 0.1% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab125244 is purified by protein A affinity chromatography from TCS -
Clonality
Monoclonal -
Clone number
RF5R -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-RFP antibody [RF5R] (ab125244)All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
Lane 3 : HEK293 cell lysate, DsRed-transfected
Developed using the ECL technique.
Predicted band size: 26 kDa
