Anti-RFP antibody (ab124754)
Key features and details
- Rabbit polyclonal to RFP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rabbit, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-RFP antibody
See all RFP primary antibodies -
Description
Rabbit polyclonal to RFP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to RFP. Corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RFP antibody (ab124754)All lanes : Anti-RFP antibody (ab124754) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 3 : CHO-K1 (chinese hamster ovary cell line) cell lysate at 10 µg
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate at 10 µg
Lane 5 : A431 (human epidermoid carcinoma cell line) cell lysate at 10 µg
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 10 µg
Lane 8 : E-coli HCP Control at 10 µg
Lane 9 : FLAG Positive Control Lysate at 10 µg
Lane 10 : Red Fluorescent Protein at 0.05 µg
Lane 11 : Green Fluorescent Protein at 0.05 µg
Lane 12 : Glutathione-S-Transferase Protein at 0.05 µg
Lane 13 : Maltose Binding Protein at 0.05 µg
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Western blot - Anti-RFP antibody (ab124754)All lanes : Anti-RFP antibody (ab124754) at 1/1000 dilution
Lane 1 : RFP with Blocking buffer
Lane 2 : Human IgG with Blocking buffer
Lane 3 : Goat IgG with Blocking buffer
Lane 4 : Mouse IgG with Blocking buffer
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : Rabbit peroxidase conjugate at 1/40000 dilution
Observed band size: 30 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (ab124754)PFA-fixed DsRed transgenic mouse retina tissue stained for RFP using ab124754 at 1/100 dilution in immunohistochemical analysis.
Blocking: 3% BSA, 0.3% Triton.
Secondary antibody: Alexa488 secondary antibody at 1:10,000 for 4 hours at RT.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (ab124754)Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse lung sections, labelling RFP with ab124754 at 1/50 dilution for 1 hour at room temperature. The secondary used was a rabbit fluorescein conjugate at 1/250 incubated for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (ab124754)Immunohistochemical immunofluorescence analysis of 4% PFA fixed mouse embryonic myocardial tissue, labelling RFP with ab124754 at a 1/50 dilution for 1 hour at room temperature. The secondary used was a a rabbit fluorescein conjugate at a 1/250 dilution for 1 hour at room temperature. Counterstain is an expression of RFP in red Tomato transgenic mice.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFP antibody (ab124754)Immunohistochemical immunofluorescence analysis of 0.5% PFA fixed Rabbit Hair folicle section labelling RFP using ab124754 at a dilution of 10 ug/mL for 1 hour at room temperature. The secondary used was a blue rabbit fluorescein conjugate at a 1/10,000 for 45 mins at room temperature. A counterstain against HOP-derived cells was used (red).
