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Signal Transduction Antibodies rfp

Anti-RFP antibody (ab152123)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-RFP antibody (ab152123)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RFP
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RFP antibody
    See all RFP primary antibodies
  • Description

    Rabbit polyclonal to RFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length RFP.

  • Positive control

    • RFP-transfected HeLa cells; RFP-transfected 293T lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • RFP

Images

  • Western blot - Anti-RFP antibody (ab152123)
    Western blot - Anti-RFP antibody (ab152123)
    All lanes : Anti-RFP antibody (ab152123) at 1/10000 dilution

    Lane 1 : Non-transfected 293T whole cell lysate
    Lane 2 : GFP-transfected 293T whole cell lysate
    Lane 3 : RFP-transfected 293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 27 kDa



    12% SDS PAGE
  • Immunoprecipitation - Anti-RFP antibody (ab152123)
    Immunoprecipitation - Anti-RFP antibody (ab152123)
    Immunoprecipitation of RFP-tagged protein.
    HeLa transfected lysates were subjected to immunoprecipitation using:
    Lane 1: 15 µg of HeLa transfected lysate
    Lane 2: normal rabbit IgG
    Lane 3: 2.5 µg ab152123
    Detection ab152123 at 1/10000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab152123)
    Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (ab152123)
    Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123: (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RFP antibody (ab152123)

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    Applications: WB

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    Applications: WB

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    Applications: ICC/IF, WB

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    Applications: WB

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