Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HA tag antibody [HA.C5] (ab18181)

Key features and details

  • Mouse monoclonal [HA.C5] to HA tag
  • Suitable for: WB, ICC, IP, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG3

Overview

  • Product name

    Anti-HA tag antibody [HA.C5]
    See all HA tag primary antibodies

  • Description

    Mouse monoclonal [HA.C5] to HA tag

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide from influenza hemagglutinin epitope:

    YPYDVPDYA

    conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Purified from TCS

  • Clonality

    Monoclonal

  • Clone number

    HA.C5

  • Isotype

    IgG3

  • Research areas

Images

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181) This image is courtesy of an anonymous Abreview
    All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

    Lanes 1-2 : HEK293 whole cell lysate - transfected
    Lane 3 : HEK293 whole cell lysate - untransfected

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181) This image is courtesy of an Abreview submitted by Harendra Chahar
    ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
  • Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
    Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
  • Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
    Western blot - Anti-HA tag antibody [HA.C5] (ab18181) This image is courtesy of an anonymous Abreview
    All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

    Lane 1 : WCE from cell line transfected for HA-tagged protein
    Lane 2 : WCE from a cell line transfected with empty vector

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-mouse IgG (H+L)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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