Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)
![Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)](https://www.abcam.com/ps/products/81/ab81656/Images/ab81656-457460-alkaline-phosphatase-anti-ha-tag-antibody-16b12-western-blot.jpg "Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)")
Key features and details
- Alkaline Phosphatase Mouse monoclonal [16B12] to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG1
Overview
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Product name
Alkaline Phosphatase Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
Alkaline Phosphatase Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
Alkaline Phosphatase -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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Epitope
The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)All lanes : Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656) at 1/10000 dilution
Lane 1 : 60 kDa HA-tagged protein at 0.3 µg
Lane 2 : 60 kDa HA-tagged protein at 0.01 µg
Lane 3 : 60 kDa HA-tagged protein at 0.03 µg
Lane 4 : 60 kDa HA-tagged protein at 0.001 µg
Lane 5 : 60 kDa HA-tagged protein at 0.003 µgImaged using stabilized Alkaline Phosphatase substrate.
