HRP Anti-HA tag antibody (ab128131)
Key features and details
- HRP Rabbit polyclonal to HA tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-HA tag antibody
See all HA tag primary antibodies -
Description
HRP Rabbit polyclonal to HA tag -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Human HA tag conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HEK293 (Human embryonic kidney cell line) - expressing HA tag protein; E. coli Positive Control (Escherichia coli ).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-HA tag antibody (ab128131)All lanes : HRP Anti-HA tag antibody (ab128131) at 1/5000 dilution
Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48,55 kDa
Observed band size: 48,55 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Abcam recommends using 3% milk as the blocking agent.
