Anti-HA tag antibody [4C12] (ab1424)
![Anti-HA tag antibody [4C12] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/HA-tag-Primary-antibodies-ab1424-6.jpg "Anti-HA tag antibody [4C12] (ab1424)")
Key features and details
- Mouse monoclonal [4C12] to HA tag
- Suitable for: ICC/IF, ChIP, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HA tag antibody [4C12]
See all HA tag primary antibodies -
Description
Mouse monoclonal [4C12] to HA tag -
Host species
Mouse -
Specificity
The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data). -
Tested applications
Suitable for: ICC/IF, ChIP, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Positive control
- WB: Rab6-HA transfected HEK-293T whole cell lysate. IP: Rab6-HA transfected HEK-293T whole cell lysate. ICC/IF: Baby hamster kidney cell expressing HA-tag CFTR protein. ChIP: Xenopus laevis oocytes injected with mRNA for HA-tagged human BORIS.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
4C12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HA tag antibody [4C12] (ab1424) This image is courtesy of an anonymous AbreviewAnti-HA tag antibody [4C12] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg
Secondary
HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 seconds
Blocked with 5% milk for 30 min at 25°C -
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [4C12] (ab1424) This image was kindly supplied as part of the review submitted by one of our customers.This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
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![Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424-302929-1424.jpg)
Immunoprecipitation - Anti-HA tag antibody [4C12] (ab1424) Data courtesy of an anonymous AbReviewRecombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
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![ChIP - Anti-HA tag antibody [4C12] (ab1424)](https://www.abcam.com/ps/products/1/ab1424/Images/ab1424_2.jpg)
ChIP - Anti-HA tag antibody [4C12] (ab1424)Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
