Anti-Elafin/ESI antibody (ab46774)
Key features and details
- Rabbit polyclonal to Elafin/ESI
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Elafin/ESI antibody
See all Elafin/ESI primary antibodies -
Description
Rabbit polyclonal to Elafin/ESI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Elafin/ESI (internal sequence).
(Peptide available asab46834) -
General notes
Previously labelled as Elafin/Skalp.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Elafin/ESI antibody (ab46774)All lanes : Anti-Elafin/ESI antibody (ab46774) at 1/1000 dilution
Lane 1 : recombinant Human Elafin at 0.05 µg
Lane 2 : recombinant Human Elafin at 0.01 µg
Lane 3 : recombinant Human Elafin at 0.001 µg
Predicted band size: 13 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible dimer)
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Immunocytochemistry/ Immunofluorescence - Anti-Elafin/ESI antibody (ab46774)ICC/IF image of ab46774 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46774, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
