Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383)
![Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383)](https://www.abcam.com/ps/products/45/ab45383/Images/ab45383-57558-anti-gsk3-alpha-beta-phospho-y216-y279-antibody-m132-western-blot.gif "Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383)")
Key features and details
- Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279)
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132]
See all GSK3 (alpha + beta) primary antibodies -
Description
Mouse monoclonal [M132] to GSK3 (alpha + beta) (phospho Y216 + Y279) -
Host species
Mouse -
Specificity
This antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3ß and GSK-3a -
Tested Applications & Species
See all applications and species dataApplication Species WB Rabbit
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Immunogen
A phospho-GSK-3ß (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3ß.
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Positive control
- Pervanadate treated rabbit fibroblasts and treated human SKN-SH and A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M132 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383)Lanes 1-2 : anti-GSK-3ß
Lanes 3-4 : Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [M132] (ab45383) at 1/1000 dilution
Lanes 1 & 3 : rabbit spleen fibroblasts serum starved for 2 hrs
Lanes 2 & 4 : rabbit spleen fibroblasts treated with pervanadate (1 mM) for 30 minutes
Predicted band size: 52 kDa
Observed band size: 46,50 kDa why is the actual band size different from the predicted?
