Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR933Y] to GSK3 (alpha + beta) (phospho Y216 + Y279) - BSA and Azide free
  • Suitable for: IHC-P, Dot blot, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free
    See all GSK3 (alpha + beta) primary antibodies

  • Description

    Rabbit monoclonal [EPR933Y] to GSK3 (alpha + beta) (phospho Y216 + Y279) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Dot blot, IP, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human thyroid carcinoma, human ovarian carcinoma.

  • General notes

    Ab239862 is the carrier-free version of ab68476. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239862 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GSK3 (alpha + beta) with purified ab68476 at 1/2000. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. ab209101, a Rabbit specific IHC polymer detection kit HRP/DAB was used. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    Positive staining on human thyroid carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab68476 for 30 minutes at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling GSK3 (alpha + beta) with purified ab68476 at 1/2000. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. ab209101, a Rabbit specific IHC polymer detection kit HRP/DAB was used. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    Positive staining on human ovarian carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab68476 for 30 minutes at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling GSK3 (alpha + beta) with Purified ab68476 at 1:50 dilution (5.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

  • Immunoprecipitation - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Immunoprecipitation - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    GSK3 (alpha + beta) (phospho Y216 + Y279) was immunoprecipitated from 10μg HeLa (human cervix adenocarcinoma) whole cell lysate with ab68476 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab68476 at 1/200 dilution (9 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1 (Input): HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysate 10μg
    Lane 2 (+): HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68476 in HeLa (human cervix adenocarcinoma) treated with 1uM staurosporine for 4h whole cell lysate

    Exposure Time: 30 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

  • Dot Blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Dot Blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    Dot Blot analysis of Lane 1: Human GSK3 (alpha + beta) (pY216 + pY279) phospho peptide and Lane 2: Human GSK3 (alpha + beta) non-phospho peptide labeling GSK3 (alpha + beta) (phospho Y216 + Y279) with ab68476 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

    Human brain tissue stained with unpurified ab68476 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68476).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)
    Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [EPR933Y] - BSA and Azide free (ab239862)

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