Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y237] to GRB2
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-GRB2 antibody [Y237]
See all GRB2 primary antibodies
Description

Rabbit monoclonal [Y237] to GRB2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Positive control
- WB: HEK293, PC-12 and NIH/3T3 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y237
Isotype

IgG
Research areas

Western blot - Anti-GRB2 antibody [Y237] (ab32037)

All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) cell lysate
Lane 3 : HEK293 (human epithelial cell line from embryonic kidney) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking/Dilution buffer : 5% NFDM/TBST

Exposure time : 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)

Immunocytochemistry/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) cells staining GRB2 with ab32037 at 1/200 dilution (green), followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (blue). Confocal image showing nuclear and weak cytoplasmic staining in HeLa cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution (red). DAPI was used as a nuclear counterstain.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is [secondary antibody name] ab150077 at 1/1000 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)

Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)

Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GRB2 (red) with ab32037 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-GRB2 antibody [Y237] (ab32037) This image is a courtesy of Anonymous Abreview

Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 25 kDa
Observed band size: 24 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute
See Abreview
Western blot - Anti-GRB2 antibody [Y237] (ab32037)

Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Recombinant Human GRB2 protein (ab85917) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Exposure time: 2 minutes
Anti-GRB2 antibody [Y237] (ab32037)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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