Anti-GRB2 antibody (ab79876)
Key features and details
- Rabbit polyclonal to GRB2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRB2 antibody
See all GRB2 primary antibodies -
Description
Rabbit polyclonal to GRB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human GRB2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88164) -
Positive control
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Hela; Hek293; A431; A549; Ramos; HepG2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenCowPigMacaque monkeyOrangutanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 386572 Chicken
- Entrez Gene: 535298 Cow
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100173972 Orangutan
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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Western blot - Anti-GRB2 antibody (ab79876)All lanes : Anti-GRB2 antibody (ab79876) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
GRB2 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab79876 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-GRB2 antibody (ab79876)All lanes : Anti-GRB2 antibody (ab79876) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
GRB2 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted.
