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Anti-CTAG1B antibody [SP349] (ab223498)

Key features and details

  • Rabbit monoclonal [SP349] to CTAG1B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CTAG1B antibody [SP349]
    See all CTAG1B primary antibodies

  • Description

    Rabbit monoclonal [SP349] to CTAG1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human CTAG1B.
    Database link: P78358

  • Positive control

    • IHC-P: Human testis, and Human gastric carcinoma tissue; WB: NY-ESO-1 or LAGE-2 transfected HEK-293 cell lysates.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    SP349

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)
    Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)
    Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified ab223498 at 1:380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)

    Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using ab223498 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-CTAG1B antibody [SP349] (ab223498)
    Western blot - Anti-CTAG1B antibody [SP349] (ab223498)
    All lanes : Anti-CTAG1B antibody [SP349] (ab223498) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with LAGE-1 cell lysate.
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) CTAG1B isoform1a transfected cell lysate.
  • Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)
    Flow Cytometry - Anti-CTAG1B antibody [SP349] (ab223498)

    Flow cytometric analysis of ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (ab223498)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with ab223498 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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