Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13445] to GOLPH4/GPP130 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free
    See all GOLPH4/GPP130 primary antibodies

  • Description

    Rabbit monoclonal [EPR13445] to GOLPH4/GPP130 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250522 is the carrier-free version of ab181849 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250522 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as GOLPH4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)
    Flow Cytometry - Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)
    This data was developed using ab181849, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling GOLPH4/GPP130 with ab181849 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
  • Western blot - Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)
    Western blot - Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)
    All lanes : Anti-GOLPH4/GPP130 antibody [EPR13445] (ab181849) at 1/20000 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 82 kDa



    This data was developed using ab181849, the same antibody clone in a different buffer formulation.

     

  • Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)
    Anti-GOLPH4/GPP130 antibody [EPR13445] - BSA and Azide free (ab250522)

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