Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (citrulline R26) antibody [EPR20606] - BSA and Azide free (ab242414)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20606] to Histone H3 (citrulline R26) - BSA and Azide free
Suitable for: Dot blot, ELISA, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (citrulline R26) antibody [EPR20606] - BSA and Azide free
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR20606] to Histone H3 (citrulline R26) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Dot blot, ELISA, WBmore details
Species reactivity

Reacts with: Mouse, Human
Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Dot blot: Histone H3 (citrulline R26) peptide (aa25-36).
General notes
Ab242414 is the carrier-free version of ab212082. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242414 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20606
Isotype

IgG

ELISA - Anti-Histone H3 (citrulline R26) antibody [EPR20606] - BSA and Azide free (ab242414)

ELISA using ab212082 labeling Histone H3 (Citrulline R26), Histone H3R26 unmodified, Histone H3 (Citrulline R17), Histone H3 (Citrulline R8), Histone H3 (Citrulline R2) and Histone H3R2/8/17 unmodified at 1 µg/ml. ab212082 was used at a range between 0 -1 µg/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) was used as secondary antibody at 1/2500 dilution.
ab212082 cross binds H3R8Cit at 14%.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212082)
Dot Blot - Anti-Histone H3 (citrulline R26) antibody [EPR20606] - BSA and Azide free (ab242414)

Dot blot analysis of Histone H3 (citrulline R26) labeled with ab212082 at 1/1000 dilution.
Lane 1: Histone H3 (citrulline R26) peptide (aa25-36)
Lane 2: Histone H3 (Citrulline R17) peptide (aa1-21)
Lane 3: Histone H3 (Citrulline R8) peptide (aa1-21)
Lane 4: Histone H3 (Citrulline R2) peptide (aa1-21)
Lane 5: Histone H3 peptide (aa1-21)
Lane 6: Histone H3 peptide (aa19-36)
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212082)
Anti-Histone H3 (citrulline R26) antibody [EPR20606] - BSA and Azide free (ab242414)

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