Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates
      IHC-P: Human testis tissue

  • General notes

    ab218120 is the carrier-free version of ab133525 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab218120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution

    Lane 1 : Molt 4 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133525, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    This data was developed using ab133525, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    OI-RD Scanning - Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    This data was developed using ab133525, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)
    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] - BSA and Azide free (ab218120)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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