Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5288] to GGT1/GGT - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free
    See all GGT1/GGT primary antibodies

  • Description

    Rabbit monoclonal [EPR5288] to GGT1/GGT - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247856 is the carrier-free version of ab109427 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247856 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as GGT1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 50-60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109427, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    This data was developed using ab109427, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GGT1/GGT with purified ab109427 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    Western blot - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    All lanes : Anti-GGT1/GGT antibody [EPR5288] (ab109427) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa



    This data was developed using ab109427, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    This data was developed using ab109427, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GGT1/GGT with unpurified ab109427 at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)
    Anti-GGT1/GGT antibody [EPR5288] - BSA and Azide free (ab247856)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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