Anti-GAPDH antibody - Loading Control (ab70700)
Key features and details
- Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
Rabbit polyclonal to GAPDH - Loading Control -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Synthetic peptide within Mouse GAPDH aa 300-335 (C terminal). The exact sequence is proprietary.
Database link: P16858 -
Positive control
- Whole cell lysate from mouse NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody - Loading Control (ab70700)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse squamous cell carcinoma tissue labelling GAPDH with ab70700 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-GAPDH antibody - Loading Control (ab70700)All lanes : Anti-GAPDH antibody - Loading Control (ab70700) at 0.04 µg/ml
Lane 1 : Whole cell lysate from mouse NIH3T3 cells. at 50 µg
Lane 2 : Whole cell lysate from mouse NIH3T3 cells. at 15 µg
Lane 3 : Whole cell lysate from mouse NIH3T3 cells. at 5 µg
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
