Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [FF26A] to GAPDH - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GAPDH antibody [FF26A] - BSA and Azide free
    See all GAPDH primary antibodies

  • Description

    Mouse monoclonal [FF26A] to GAPDH - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus within Human GAPDH. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IP: HepG2 and MDA-MB-231 whole cell lysate. WB: Human brain, heart, kidney and spleen tissue lysate. HeLa, HepG2, Jurkat, A549 and MDA-MB-231 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255970 is the carrier-free version of ab59164. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Western blot - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)
    Western blot - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)
    All lanes : Anti-GAPDH antibody [FF26A] (ab59164) at 0.441 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
    Lane 4 : A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 6 : Human brain tissue lysate at 10 µg
    Lane 7 : Human heart tissue lysate at 10 µg
    Lane 8 : Human kidney tissue lysate at 10 µg
    Lane 9 : Human spleen tissue lysate at 10 µg

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-5: 7 secs; Lanes 6-9: 10 secs.

    This image was produced using the same antibody clone but in a different formulation ab59164, PBS, sodium azide, glycerol and BSA.

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)
    Immunoprecipitation - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)

    GADPH was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab59164 at 14.7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/5000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
    Lane 1: HepG2 whole cell lysate 10µg (Input).
    Lane 2: ab59164 IP in HepG2 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

    This image was produced using the same antibody clone but in a different formulation ab59164, PBS, sodium azide, glycerol and BSA.

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)
    Immunoprecipitation - Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)

    GADPH was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab59164 at 14.7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
    Lane 1: MDA-MB-231 whole cell lysate 10µg (Input).
    Lane 2: ab59164 IP in MDA-MB-231 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in MDA-MB-231 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

    This image was produced using the same antibody clone but in a different formulation ab59164, PBS, sodium azide, glycerol and BSA.

  • Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)
    Anti-GAPDH antibody [FF26A] - BSA and Azide free (ab255970)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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