Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)
![Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)](https://www.abcam.com/ps/products/236/ab236547/Images/ab236547-313141-725.jpg "Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20832] to FNIP1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-FNIP1 antibody [EPR20832] - BSA and Azide free
See all FNIP1 primary antibodies -
Description
Rabbit monoclonal [EPR20832] to FNIP1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Expression levels of the target protein vary with sample type and some optimisation may be required. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse muscle lysate.
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General notes
Ab236547 is the carrier-free version of ab215725. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236547 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20832 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/500 dilution
Lane 1 : Mouse muscle lysate
Lane 2 : FNIP1 knockout mouse muscle lysate
Lysates/proteins at 75 µg per lane.
Secondary
All lanes : Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutesBlocking buffer: 7% non-fat milk diluted in TBS-T.
Dilution buffer: 1% BSA diluted in TBS.
The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174).
The image was kindly provided by our collaborator Dr. Zhenji Gan, Nanjing University.
The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Expression levels of the target protein vary with sample type and some optimisation may be required.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215725).
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![Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)](https://www.abcam.com/ps/products/236/ab236547/Images/ab236547-2-benefits-of-recombinant-antibodies.png)
Anti-FNIP1 antibody [EPR20832] - BSA and Azide free (ab236547)
