Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FNIP1 antibody [EPR20832] (ab215725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20832] to FNIP1
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FNIP1 antibody [EPR20832]
    See all FNIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20832] to FNIP1

  • Host species

    Rabbit

  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse muscle and testis tissue lysates; L-929 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20832

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-FNIP1 antibody [EPR20832] (ab215725)
    Western blot - Anti-FNIP1 antibody [EPR20832] (ab215725)
    All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 1 minute; Lane 2: 70 seconds.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174). The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Lane 2 was developed using a high sensitivity ECL substrate. Expression levels of the target protein vary with sample type and some optimisation may be required

  • Western blot - Anti-FNIP1 antibody [EPR20832] (ab215725)
    Western blot - Anti-FNIP1 antibody [EPR20832] (ab215725)
    All lanes : Anti-FNIP1 antibody [EPR20832] (ab215725) at 1/500 dilution

    Lane 1 : Mouse muscle lysate
    Lane 2 : FNIP1 knockout mouse muscle lysate

    Lysates/proteins at 75 µg per lane.

    Secondary
    All lanes : Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/7500 dilution

    Predicted band size: 130 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?


    Exposure time: 15 minutes


    Blocking buffer: 7% non-fat milk diluted in TBS-T.

    Dilution buffer: 1% BSA diluted in TBS.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 17028174).

    The image was kindly provided by our collaborator Dr. Zhenji Gan, Nanjing University.

    The specific Fnip1 band is around 150 kDa; the other bands are non-specific. Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Anti-FNIP1 antibody [EPR20832] (ab215725)
    Anti-FNIP1 antibody [EPR20832] (ab215725)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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