Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free
    See all ErbB2 / HER2 primary antibodies

  • Description

    Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects ErbB2 / HER2 phosphorylated at tyrosine 877.

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IF, Dot blotmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast ductal infiltrating carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt: A431 cells (+/- EGF treatment). Dot Blot: ErbB2 / HER2 (pY877) peptide.

  • General notes

    ab238437 is the carrier-free version of ab108371 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238437 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

    ab108371, at 1/250, staining Human ErbB2 / HER2 (phospho Y877) in human breast ductal infiltrating carcinoma tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
    Flow Cytometry - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with purified ab108371 at 1/20.

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

    ab108371, at 1/50 dilution, staining ErbB2 / HER2 (phospho Y877) in SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells by immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
    Dot Blot - Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

    Dot blot analysis of ErbB2 / HER2 (pY877) peptide (Lane 1) and ErbB2 non-phospho peptide (Lane 2) labeling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab108371)

  • Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)
    Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

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