Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102)
Key features and details
- Rabbit polyclonal to ErbB2 / HER2 (phospho Y1221 + Y1222)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit polyclonal to ErbB2 / HER2 (phospho Y1221 + Y1222) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human ErbB2/ HER2 (phospho Y1221 + Y1222) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:NLY(p)Y(p)W
Database link: P04626 -
Positive control
- WB: MDA +/-treatment withEGF, cell extract; HeLa (EGF-treated), cell extract. ICC/IF: MCF cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131102 was purified by affinity-chromatography using epitope-specific phosphopetide. Non-phospho specific antibodies were removed by chromatography using non-phosphopetide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102)All lanes : Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102) at 1/500 dilution
Lane 1 : HeLa (EGF-treated) cell extract (treated with calf intestinal phosphatase)
Lane 2 : HeLa (EGF-treated) cell extract
Predicted band size: 137 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102)Immunofluorescence analysis of methanol-fixed MCF cells labelling ErbB2 / HER2 (phospho Y1221 + Y1222) with ab131102 at 1/100 dilution.
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Western blot - Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102)All lanes : Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody (ab131102) at 1/500 dilution
Lane 1 : MDA cell extract untreated
Lane 2 : MDA (treated with EGF) cell extract
Predicted band size: 137 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
