Anti-EpCAM antibody [HEA125], prediluted (ab46714)
![Anti-EpCAM antibody [HEA125], prediluted (ab46714)](https://www.abcam.com/ps/products/46/ab46714/Images/ab46714-197421-anti-epcam-antibody-hea125-prediluted-immunofluorescence.jpg "Anti-EpCAM antibody [HEA125], prediluted (ab46714)")
Key features and details
- Mouse monoclonal [HEA125] to EpCAM, prediluted
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-EpCAM antibody [HEA125], prediluted
See all EpCAM primary antibodies -
Description
Mouse monoclonal [HEA125] to EpCAM, prediluted -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Living human colon carcinoma cell line HT-29
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Positive control
- ICC/IF: HepG2 cells.
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General notes
ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours. -
Clonality
Monoclonal -
Clone number
HEA125 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (ab46714)
ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
