Mouse DAN ELISA Kit (ab193721)
Key features and details
- Sensitivity: 35 pg/ml
- Range: 35 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DAN ELISA Kit
See all NBL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
35 pg/ml -
Range
35 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84.4 74% - 94% Plasma 91.38 83% - 99% Cell culture media 108.1 78% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s DAN mouse ELISA Kit (ab193721) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DAN in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse DAN coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DAN present in the samples. The wells are washed and biotinylated anti-mouse DAN antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DAN bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated mouse DAN detection antibody (lyophilized) 2 vials mouse DAN Standards (lyophilized) 2 vials Pre-coated mouse DAN Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- D1S1733E
- DAN
- DAN domain family member 1
see all -
Database links
- Entrez Gene: 17965 Mouse
- SwissProt: Q61477 Mouse
- Unigene: 9404 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab193721.
