Anti-EMSY antibody (ab4579)
Key features and details
- Goat polyclonal to EMSY
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EMSY antibody
See all EMSY primary antibodies -
Description
Goat polyclonal to EMSY -
Host species
Goat -
Specificity
This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human EMSY aa 1-100.
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Positive control
- MCF-7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EMSY antibody (ab4579)Lanes 1-2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml
All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesWestern blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.
Lane 2: ab4579 at 1ug/ml with 3% milk blocking.
Lane 3: ab123 at 1ug/ml with 2% BSA blocking.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Lane 1 & 2 - Exposure time: 2 min.
Lane 3 - Exposure time: 2 sec.
