Anti-EMSY antibody (ab123)
Key features and details
- Rabbit polyclonal to EMSY
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EMSY antibody
See all EMSY primary antibodies -
Description
Rabbit polyclonal to EMSY -
Host species
Rabbit -
Specificity
This antibody detects a band of the same size by Western blot as ab4579, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years after this antibody). The band detected by this antibody also runs at the same height as full length in vitro translated EMSY. This confirms that the band detected is EMSY. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human EMSY aa 100-200.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-EMSY antibody (ab123) Hughes-Davies et al, 2003
WB using EMSY antibody (ab123) demonstrating the interaction between EMSY and BRCA2 by immunoprecipitation.
Endogenous coimmunoprecipitation of EMSY and BRCA2 from unmanipulated asynchronously dividing HeLa cells.
Immunoprecipitation with an unrelated antibody (anti-GFP), preimmune serum, ab123 or anti-BRCA2 antibody was followed by Western blotting with ab123.
Neither of the unrelated antibodies could coprecipitate EMSY, whereas an EMSY signal was detected in the immunoprecipitate obtained with the anti-BRCA2 antibody. The anti-EMSY antibody could also immunoprecipitate endogenous EMSY protein.
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Immunoprecipitation - Anti-EMSY antibody (ab123)EMSY was immunoprecipitated using 0.5mg MCF7 whole cell extract, Rabbit polyclonal to EMSY diluted to 1/3,000 in RIPA buffer and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab123.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 141kDa; EMSY
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Western blot - Anti-EMSY antibody (ab123)Western blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1/500
Lane 2: ab123 at 1/500.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Secondary ab Lane 1: Rabbit polyclonal to Goat IgG H&L (HRP) ab6741 (1/5000)
Exposure time: 3 min.Secondary ab Lane 2: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed ab7090 (1/5000)
Exposure time: 10 sec.Lysate at 20
µ Lane 1 - Exposure time : 3 min.
Lane 2 - Exposure time : 10 sec.
Variability in the size at which EMSY runs by Western blot has been exper
