Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP38Y] to EGFR - BSA and Azide free
  • Suitable for: ELISA, Flow Cyt, IHC-P, WB, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-EGFR antibody [EP38Y] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [EP38Y] to EGFR - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen for this product is a synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR. After screening, clone “EP38Y” was found to recognize total EGFR and is not specific to phosphorylated-Tyr1068 EGFR. This product yielded a strong signal in western blot using A431 (human squamous carcinoma) lysate which naturally overexpresses the EGFR protein. Western blot conditions may need to be optimised for cell lines and tissues that express lower levels of endogenous EGFR. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

  • Tested applications

    Suitable for: ELISA, Flow Cyt, IHC-P, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A431 cells. WB: HeLa, Caco-2 and A431 cell lysate; Rat liver and skin lysate; Mouse lung and skin lysate; Murine macrophage lysate. IP: HeLa whole cell lysate (ab150035). Flow Cyt: A431 cells. IHC-P: Human cervical carcinoma, endometrial carcinoma, glioma, tonsil and renal cell carcinoma tissue. IHC-P: Rat skin tissue.

  • General notes

    Maxpar® is a trademark of Fluidigm Canadaab272293 is the carrier-free version of ab52894. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab52894 at 1:250 dilution (0.4 μg/ml).

    Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain.

    PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52894).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling EGFR with purified ab52894 at 1:100 dilution (0.95 μg/ml).

    Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52894).

  • Flow Cytometry - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    Flow Cytometry - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

    Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab52894.

    Cells were fixed with 4% paraformaldehyde (10 mins) and permeabilized with 90% methanol for 30 mins. Then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by ab52894 at 1/20 dilution (red) for 30 mins. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52894).

  • Immunoprecipitation - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    Immunoprecipitation - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

    ab52894 (purified) at 1:20 dilution (0.5 µg) immunoprecipitating EGFR in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate 10 µg

    Lane 2 (+): ab52894 in HeLa whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52894 in HeLa whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52894).

  • OI-RD Scanning - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    OI-RD Scanning - Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52894).

  • Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)
    Anti-EGFR antibody [EP38Y] - BSA and Azide free (ab272293)

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