Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950]
    See all Rho guanine exchange factor 16/NBR primary antibodies

  • Description

    Rabbit monoclonal [EPR9950] to Rho guanine exchange factor 16/NBR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Rho guanine exchange factor 16/NBR.
    Database link: Q5VV41

  • Positive control

    • HeLa, MCF-7 and 293T cell lysates; Mouse kidney lysates; Human cervix carcinoma tissue; Human breast tissue; HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as Rho guanine exchange factor 16

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9950

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution + Mouse kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 180 seconds


    Blocking and Diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Western blot - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    All lanes : Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunocytochemistry/ Immunofluorescence - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunofluorescent analysis of HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/100 dilution.

  • Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Flow Cytometry - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Flow cytometric analysis of permeabilized HeLa cells labeling Rho guanine exchange factor 16/NBR with ab154785 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154785 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)
    Anti-Rho guanine exchange factor 16/NBR antibody [EPR9950] (ab154785)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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