Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free
See all EFEMP1/Fibulin-3 primary antibodies
Description

Rabbit monoclonal [EPR22855-4] to EFEMP1/Fibulin-3 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra), IPmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC, Human eye, Human ovary and Human skeletal muscle lysates. IHC-P: Human breast carcinoma, Human colon carcinoma, Human colon and Human testis tissues. ICC/IF: HUVEC cells. Flow Cyt (intra): HUVEC cells. IP: HUVEC cells.
General notes
ab259998 is the carrier-free version of ab256457.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22855-4
Isotype

IgG
Research areas

Immunoprecipitation - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

EFEMP1/Fibulin-3 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab256457 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10ug

Lane 2: ab256457 IP in HUVEC whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256457 in HUVEC whole cell lysate

Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

Exposure time: 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Flow Cytometry - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Immunocytochemistry/ Immunofluorescence - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution, followed by Ab256457 anti-EFEMP1 Fibulin-3 ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Positive staining on the basement membrane in human testes. The section was incubated with ab256457 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells of human colon. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ab256457 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stromal cells of human colon carcinoma. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ab256457 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling EFEMP1/Fibulin-3 with ab256457 at 1/500 dilution (1.12 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the stroma cells of human breast carcinoma (PMID/ 19115204). The section was incubated with ab256457 for 15 mins at RT. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256457).
Anti-EFEMP1/Fibulin-3 antibody [EPR22855-4] - BSA and Azide free (ab259998)