Anti-EFEMP1/Fibulin-3 antibody (ab151976)
Key features and details
- Rabbit polyclonal to EFEMP1/Fibulin-3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EFEMP1/Fibulin-3 antibody
See all EFEMP1/Fibulin-3 primary antibodies -
Description
Rabbit polyclonal to EFEMP1/Fibulin-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human EFEMP1/Fibulin-3 aa 168-488.
Database link: Q12805 -
Positive control
- WB: HEK-293T, A431, H1299, HepG2 and HeLa whole cell lysates; rat liver tissue lysate; mouse liver tissue lysate. IHC-P: NCIN87 xenograft tissue.
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General notes
This product was previously labelled as EFEMP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa10% SDS-PAGE
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa10% SDS-PAGE
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Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/500 dilution + rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
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Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution + Mouse liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
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Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labeling EFEMP1/Fibulin-3 with ab151976 at a 1/500 dilution.
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
10% SDS PAGE