Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13381-76] to TTC4 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free
    See all TTC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13381-76] to TTC4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250418 is the carrier-free version of ab181195. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250418 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13381-76

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Western blot - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    All lanes : Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U-87 MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab181195, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Western blot - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution + MCF-7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab181195, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    This data was developed using ab181195, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Immunocytochemistry - Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    This data was developed using ab181195, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).
  • Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)
    Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free (ab250418)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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