Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21189] to Dystrophin - BSA and Azide free
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Dystrophin antibody [EPR21189] - BSA and Azide free
See all Dystrophin primary antibodies -
Description
Rabbit monoclonal [EPR21189] to Dystrophin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse cardiac muscle tissue.
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General notes
Ab230379 is the carrier-free version of ab218198. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab230379 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21189 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Immunohistochemical (Frozen sections) analysis of rat heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218198).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in smooth muscle of rat colon (PMID: 18806224) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218198).
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Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Immunohistochemical (Frozen sections) analysis of mouse heart tissue useing ab218198 (1/50 dilution) to stain Dystrophin. ab150077 AlexaFluor®488 Goat anti-Rabbit (1/500) secondary used. DAPI used as a nuclear counter stain. Heat-mediated antigen retrieval using ab94681 (Tris/EDTA buffer, pH 9.0). Fixative 4% PFA, Permeabilisation with 0.2% Triton X-100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218198).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse skeletal muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218198).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR21189] - BSA and Azide free (ab230379)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab218198 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse cardiac muscle (PMID: 24793134) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218198).
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