Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DUSP5 antibody [EPR19684] (ab200708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19684] to DUSP5
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-DUSP5 antibody [EPR19684]
    See all DUSP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR19684] to DUSP5

  • Host species

    Rabbit

  • Specificity

    Due to low expression levels, treatment with growth factors may be necessary to induce the expression of DUSP5 in certain cell lines.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate from MEFs serum starved and then treated for 2h with 20% FCS. ICC/IF: Wild-type MEF cells serum starved and then treated for 2h with 20% FCS.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19684

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution

    Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
    Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    MEF Wt and DUSP5 KO whole cell lysates used on this blot are from MEFs serum starved and then treated for 2h with 20% FCS.

    These lysates were kindly provided by Steve Keyes from Dundee University.

  • Immunocytochemistry/ Immunofluorescence - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Immunocytochemistry/ Immunofluorescence - Anti-DUSP5 antibody [EPR19684] (ab200708)

    Ab200708 staining DUSP5 in wild-type MEF cells serum starved and then treated for 2h with 20% FCS (top panel) and DUSP5 knockout MEF cells serum starved and then treated for 2h with 20% FCS (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab200708 at 1/100 dilution and anti-alpha Tubulin antibody ab7291 at 1/1000 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml. Nuclear DNA was labelled in blue with DAPI.

  • Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution

    Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 2 : Untreated MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) treated with 100 ng/ml PMA for 48 hours whole cell lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes
  • Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/5000 dilution + Human DUSP5 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant human full length protein was made in-house.

  • Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Western blot - Anti-DUSP5 antibody [EPR19684] (ab200708)
    Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution + Mouse DUSP5 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 4 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse DUSP5 full length recombinant protein contain aa1-384 with a His-Tag®. This recombinant mouse full length protein was made in-house.

  • Anti-DUSP5 antibody [EPR19684] (ab200708)
    Anti-DUSP5 antibody [EPR19684] (ab200708)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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