Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19684] to DUSP5 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human, Recombinant fragment

Overview

  • Product name

    Anti-DUSP5 antibody [EPR19684] - BSA and Azide free
    See all DUSP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR19684] to DUSP5 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Due to low expression levels, treatment with growth factors may be necessary to induce the expression of DUSP5 in certain cell lines.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
    Predicted to work with: Non human primates

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate from MEFs serum starved and then treated for 2h with 20% FCS. ICC/IF: Wild-type MEF cells serum starved and then treated for 2h with 20% FCS.

  • General notes

    ab232333 is the carrier-free version of ab200708.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19684

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
    Immunocytochemistry/ Immunofluorescence - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)

    Ab200708 staining DUSP5 in wild-type MEF cells serum starved and then treated for 2h with 20% FCS (top panel) and DUSP5 knockout MEF cells serum starved and then treated for 2h with 20% FCS (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab200708 at 1/100 dilution and anti-alpha Tubulin antibody ab7291 at 1/1000 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml. Nuclear DNA was labelled in blue with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200708).

  • Western blot - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
    Western blot - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
    All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution

    Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
    Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    MEF Wt and DUSP5 KO whole cell lysates used on this blot are from MEFs serum starved and then treated for 2h with 20% FCS.

    These lysates were kindly provided by Steve Keyes from Dundee University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200708).

  • Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
    Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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