Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
![Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)](https://www.abcam.com/ps/products/232/ab232333/Images/ab232333-382300-anti-dusp5-antibody-epr19684-bsa-and-azide-free-immunocytochemistry-immunofluorescence-mef-human.jpg "Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19684] to DUSP5 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human, Recombinant fragment
Overview
-
Product name
Anti-DUSP5 antibody [EPR19684] - BSA and Azide free
See all DUSP5 primary antibodies -
Description
Rabbit monoclonal [EPR19684] to DUSP5 - BSA and Azide free -
Host species
Rabbit -
Specificity
Due to low expression levels, treatment with growth factors may be necessary to induce the expression of DUSP5 in certain cell lines. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Non human primates
-
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human DUSP5 full length recombinant protein; Mouse DUSP5 full length recombinant protein; MEF (WT) whole cell lysate from MEFs serum starved and then treated for 2h with 20% FCS. ICC/IF: Wild-type MEF cells serum starved and then treated for 2h with 20% FCS.
-
General notes
ab232333 is the carrier-free version of ab200708.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19684 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
Ab200708 staining DUSP5 in wild-type MEF cells serum starved and then treated for 2h with 20% FCS (top panel) and DUSP5 knockout MEF cells serum starved and then treated for 2h with 20% FCS (bottom panel). The cells were fixed with 100% methanol for 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab200708 at 1/100 dilution and anti-alpha Tubulin antibody ab7291 at 1/1000 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1 hour with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 2 μg/ml. Nuclear DNA was labelled in blue with DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200708).
-
![Western blot - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)](https://www.abcam.com/ps/products/232/ab232333/Images/ab232333-308796-232333.jpg)
Western blot - Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)All lanes : Anti-DUSP5 antibody [EPR19684] (ab200708) at 1/1000 dilution
Lane 1 : MEF (Mouse embryonic fibroblast cell line) (WT) whole cell lysate
Lane 2 : MEF (Mouse embryonic fibroblast cell line) (DUSP5 KO) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
MEF Wt and DUSP5 KO whole cell lysates used on this blot are from MEFs serum starved and then treated for 2h with 20% FCS.
These lysates were kindly provided by Steve Keyes from Dundee University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200708).
-
![Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)](https://www.abcam.com/ps/products/232/ab232333/Images/ab232333-3-benefits-of-recombinant-antibodies.png)
Anti-DUSP5 antibody [EPR19684] - BSA and Azide free (ab232333)
