Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DLK-1 antibody [EPR19830] (ab210471)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19830] to DLK-1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat

Product name

Anti-DLK-1 antibody [EPR19830]
See all DLK-1 primary antibodies
Description

Rabbit monoclonal [EPR19830] to DLK-1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse placenta and embryo lysates. IHC-P: Mouse cerebrum and E14.5 embryo tissues; Rat cerebrum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Rat
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19830
Isotype

IgG
Research areas

Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)

All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution

Lane 1 : Mouse placenta lysate
Lane 2 : DLK-1-knockout mouse placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)

All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/5000 dilution

Lane 1 : Mouse placenta lysate at 0.005 µg
Lane 2 : Mouse embryo lysate at 5 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 41 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-DLK-1 antibody [EPR19830] (ab210471)

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