Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DLK-1 antibody [EPR19830] (ab210471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19830] to DLK-1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-DLK-1 antibody [EPR19830]
    See all DLK-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19830] to DLK-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta and embryo lysates. IHC-P: Mouse cerebrum and E14.5 embryo tissues; Rat cerebrum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
    Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
    All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution

    Lane 1 : Mouse placenta lysate
    Lane 2 : DLK-1-knockout mouse placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

    The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
    Western blot - Anti-DLK-1 antibody [EPR19830] (ab210471)
    All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/5000 dilution

    Lane 1 : Mouse placenta lysate at 0.005 µg
    Lane 2 : Mouse embryo lysate at 5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] (ab210471)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-DLK-1 antibody [EPR19830] (ab210471)
    Anti-DLK-1 antibody [EPR19830] (ab210471)

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