Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
![Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)](https://www.abcam.com/ps/products/230/ab230380/Images/ab230380-4-ab2104715210471IHC3.jpg "Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19830] to DLK-1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-DLK-1 antibody [EPR19830] - BSA and Azide free
See all DLK-1 primary antibodies -
Description
Rabbit monoclonal [EPR19830] to DLK-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse placenta lysate.
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General notes
Ab230380 is the carrier-free version of ab210471. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab230380 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19830 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)](https://www.abcam.com/ps/products/230/ab230380/Images/ab230380-3-ab2104714210471IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).
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![Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)](https://www.abcam.com/ps/products/230/ab230380/Images/ab230380-298073-23038033.jpg)
Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution
Lane 1 : Mouse placenta lysate
Lane 2 : DLK-1-knockout mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 32 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).
The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)](https://www.abcam.com/ps/products/230/ab230380/Images/ab230380-297975-230380.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)](https://www.abcam.com/ps/products/230/ab230380/Images/ab230380-5-benefits-of-recombinant-antibodies.png)
Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
