Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19830] to DLK-1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-DLK-1 antibody [EPR19830] - BSA and Azide free
    See all DLK-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19830] to DLK-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta lysate.

  • General notes

    Ab230380 is the carrier-free version of ab210471. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230380 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of rat cerebrum (PMID: 19515692) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse E14.5 embryo (PMID: 16456855) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).

  • Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    Western blot - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    All lanes : Anti-DLK-1 antibody [EPR19830] (ab210471) at 1/1000 dilution

    Lane 1 : Mouse placenta lysate
    Lane 2 : DLK-1-knockout mouse placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 32 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression pattern is consistent with the literature (PMID: 21419176 and PMID: 19247431).

    The tissue lysates were kindly provided by our collaborator Prof. Anne Ferguson-Smith, Cambridge University.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLK-1 with ab210471 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in the substantia nigra and ventral tegmental area (arrow) of mouse cerebrum (PMID: 19515692). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210471).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)
    Anti-DLK-1 antibody [EPR19830] - BSA and Azide free (ab230380)

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