Anti-DLK-1 antibody (ab21682)
Key features and details
- Rabbit polyclonal to DLK-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-DLK-1 antibody
See all DLK-1 primary antibodies -
Description
Rabbit polyclonal to DLK-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human DLK-1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23442) -
Positive control
- This antibody gave a positive signal in E18.5 Mouse Muscle Wild Type Tissue Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatCowHumanApplication Abreviews Notes WB 1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
Notes WB
1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
Target
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Function
May have a role in neuroendocrine differentiation. -
Tissue specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
Sequence similarities
Contains 6 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281117 Cow
- Entrez Gene: 8788 Human
- Entrez Gene: 13386 Mouse
- Entrez Gene: 114587 Rat
- Omim: 176290 Human
- SwissProt: P80370 Human
- SwissProt: Q09163 Mouse
- Unigene: 533717 Human
see all -
Alternative names
- Adipocyte differentiation inhibitor protein antibody
- Brevideltin antibody
- Delta like 1 homolog antibody
see all
Images
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Western blot - Anti-DLK-1 antibody (ab21682)All lanes : Anti-DLK-1 antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))
Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThis blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
References (19)
ab21682 has been referenced in 19 publications.
- Dokoshi T et al. Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection. Cell Rep 30:61-68.e4 (2020). PubMed: 31914398
- Grassi ES et al. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene 39:4028-4044 (2020). PubMed: 32205867
- Yang W et al. Adiponectin promotes preadipocyte differentiation via the PPAR? pathway. Mol Med Rep 17:428-435 (2018). PubMed: 29115433
- Cast A et al. C/EBPa-dependent preneoplastic tumor foci are the origin of hepatocellular carcinoma and aggressive pediatric liver cancer. Hepatology 67:1857-1871 (2018). PubMed: 29159818
- Villani R et al. Dominant-negative Sox18 function inhibits dermal papilla maturation and differentiation in all murine hair types. Development 144:1887-1895 (2017). PubMed: 28512199
Images
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Western blot - Anti-DLK-1 antibody (ab21682)All lanes : Anti-DLK-1 antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))
Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45,48 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThis blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
