Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)
![Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)](https://www.abcam.com/ps/products/124/ab124814/Images/ab124814-138730-anti-dihydrofolate-reductase-dhfr-antibody-epr5285-western-blot.jpg "Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]
See all Dihydrofolate reductase (DHFR) primary antibodies -
Description
Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 150-250. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, Y-79, 293T, Mouse spleen and Rat kidney lysates; Human cervical carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5285 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Y-79 cell lysates
Lane 4 : 293T cell lysates
Lane 5 : Mouse spleen lysates
Lane 6 : Rat kidney lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)](https://www.abcam.com/ps/products/124/ab124814/Images/ab124814-138729-anti-dihydrofolate-reductase-dhfr-antibody-epr5285-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)](https://www.abcam.com/ps/products/124/ab124814/Images/ab124814-3-benefits-of-recombinant-antibodies.png)
Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814)
