Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)
Key features and details
- Rabbit polyclonal to Dihydrofolate reductase (DHFR)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dihydrofolate reductase (DHFR) antibody
See all Dihydrofolate reductase (DHFR) primary antibodies -
Description
Rabbit polyclonal to Dihydrofolate reductase (DHFR) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide within Human Dihydrofolate reductase (DHFR) aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: P00374 -
Positive control
- Breast Carcinoma
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)Formalin-fixed, paraffin-embedded section of Human breast carcinoma stained with ab82171 at 1/50 dilution. Incubate primary antibody (ab82171) for 30 minutes at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Dihydrofolate reductase (DHFR) antibody (ab82171)ICC/IF image of ab82171 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82171, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
